A NOVEL HUMAN HOMOLOG OF A DEAD-BOX RNA HELICASE FAMILY

Putative cDNA clones for a nuclear antigen that crossreacts with anti- human aldolase A monoclonal antibody MAb1A2 were isolated from the HeL a lambda gt11 cDNA library and a candidate clone (clone 3) was analyze d. The cDNA has an open reading frame (ORF) of 1,317 bp encoding a nov el RNA helicase belonging to the DEAD RNA helicase family. The ORF als o contains a nuclear targeting signal and the epitope for MAb1A2, The putative RNA helicase has sequence similarity to Escherichia coli RNA helicase DEAD, mouse translation factor eIF-4A, and human p68 and p54. (C) 1994 Academic Press, Inc.