TIME-RESOLVED IMMUNOFLUOROMETRIC ASSAY OF TOTAL RENIN IN PLASMA AND FOLLICULAR-FLUID

We developed a new time-resolved immunofluorometric assay (TR-IFMA) us ing two monoclonal antibodies for the total renin measurement in human plasma and follicular fluid. No conversion of prorenin to renin was n eeded because the assay detected both renin and prorenin. The detectio n limit of the assay was 10 ng/L and the linear range was 10-25 000 ng /L. Within-assay precision (CV) was 15-8% at renin concentrations of 5 0-12 200 ng/L. Between-assay precision was 19-3% at concentrations of 100-18 000 ng/L. Analytical recovery of added renin was 85-104% (n = 5 ) in plasma samples and 104-119% (n = 3) in follicular fluids. For pla sma, the reference interval was 78-262 ng/L in men (n = 44) and 36-226 ng/L in women (n = 43).