INTERNALLY CONSISTENT LIBRARIES OF FLUOROGENIC SUBSTRATES DEMONSTRATETHAT KEX2 PROTEASE SPECIFICITY IS GENERATED BY MULTIPLE MECHANISMS

Kex2 protease from the yeast Saccharomyces cerevisiae is the prototype for a family of eukaryotic proprotein processing proteases. To clarif y understanding of the interactions responsible for substrate recognit ion in this family of enzymes, we have carried out a systematic examin ation of Kex2 substrate specificity using internally consistent sets o f substrates having substitutions at only one or two positions. We exa mined Kex2 sequence recognition for residues at P-3, P-2, and P-1 usin g two types of fluorogenic peptide substrates, peptidyl-methylcoumarin amides and internally quenched substrates in which cleavage occurs at an actual peptide bond. Kinetic analysis of the two sets of substrates gave comparable data on specificity at these three positions. For the best substrate sequences, high catalytic constants (k(cat)/K-M) of (2 -5) x 10(7) M(-1) s(-1) were seen for cleavage of both peptidyl-methyl coumarinamides and peptide bonds. While no evidence for positive inter actions with the P-3 residue emerged, Kex2 was found to discriminate a gainst at least one residue, Asp, at this position. Specificity at P-2 was shown to rely primarily on recognition of a positive charge, alth ough steric constraints on the P-2 side chain were also apparent. Kex2 was demonstrated to be exquisitely selective for Arg at P-1. Substitu tions with similar charge (Lys, ornithine) or similar hydrogen-bonding capability (citrulline) do not confer efficient catalysis. Comparison of otherwise identical substrates having either Arg or citrulline at P-1 showed that the positive charge of the Arg guanidinium group stabi lizes the transition state by approximately 6.8 kcal/mol.