COMPARISON OF SELECTIVE AGENTS FOR USE WITH THE SELECTABLE MARKER GENE BAR IN MAIZE TRANSFORMATION

Citation
Bk. Dennehey et al., COMPARISON OF SELECTIVE AGENTS FOR USE WITH THE SELECTABLE MARKER GENE BAR IN MAIZE TRANSFORMATION, Plant cell, tissue and organ culture, 36(1), 1994, pp. 1-7
Citations number
19
Categorie Soggetti
Plant Sciences
ISSN journal
01676857
Volume
36
Issue
1
Year of publication
1994
Pages
1 - 7
Database
ISI
SICI code
0167-6857(1994)36:1<1:COSAFU>2.0.ZU;2-7
Abstract
The effectiveness of four phosphinothricin (PPT)-based selective agent s were evaluated for use in maize transformation: glufosinate, bialaph os, Basta(R) and Herbiace(R). Glufosinate and its commercial formulati on, Basta(R), were less effective in controlling growth of non-transge nic corn callus than the tripeptide, bialaphos, or its commercial form ulation, Herbiace(R). Addition of 25 mM L-proline had no significant e ffect on selection when using bialaphos. However, when L-proline was i ncluded with the selective agent glufosinate, selection was inhibited and callus growth was enhanced. At four weeks, callus growth on 0.3, 1 .0 and 3.0 mg l-1 glufosinate in the presence of proline was 76, 43, a nd 21% of control growth, respectively, and in the absence of proline was only 32, 9, and 6% of control growth. Optimized selection protocol s for Basta(R) and bialaphos yielded comparable numbers of transforman ts. Using these protocols, fertile transgenic plants were regenerated from transformed callus cultures.