Ml. Suardi et al., IN-VITRO CULTURES OF SOLANUM-MALACOXYLON SENDT - NUTRITIONAL-REQUIREMENTS AND STEROL PRODUCTION, Plant cell, tissue and organ culture, 36(1), 1994, pp. 9-14
Callus cultures were established from hypocotyl, root and leaf explant
s of Solanum malacoxylon. The growth rate of calli was evaluated on Mu
rashige and Skoog medium as well as Gamborg's B5 medium. Sterols were
isolated from calli grown on both media however the B5 proved to be mo
re effective in promoting the production of these metabolites. No calc
itriol was found in the cultures. Feeding experiments with vitamin D3
were scarcely effective in promoting the production of vitamin D3 hydr
oxylated metabolites.