BINDING OF RIBULOSE 1,5-BISPHOSPHATE TO THE NONCATALYTIC SITES OF RIBULOSE 1,5-BISPHOSPHATE CARBOXYLASE OXYGENASE AND ITS METABOLIC IMPLICATIONS/

Citation
A. Yokota et al., BINDING OF RIBULOSE 1,5-BISPHOSPHATE TO THE NONCATALYTIC SITES OF RIBULOSE 1,5-BISPHOSPHATE CARBOXYLASE OXYGENASE AND ITS METABOLIC IMPLICATIONS/, Plant and Cell Physiology, 35(2), 1994, pp. 317-321
Citations number
27
Categorie Soggetti
Plant Sciences
Journal title
ISSN journal
00320781
Volume
35
Issue
2
Year of publication
1994
Pages
317 - 321
Database
ISI
SICI code
0032-0781(1994)35:2<317:BOR1TT>2.0.ZU;2-D
Abstract
This is the first report showing that ribulose bisphosphate carboxylas e/oxygenase has the non-catalytic sites to bind ribulose bisphosphate (RuBP). A plot of the binding number against the RuBP concentration in the equilibrium binding assay gave a bumpy curve with an intermediate plateau at 0.3 to 0.5 mM RuBP. The binding was saturated with 1.5 mM RuBP. The concentrations of free and binding forms of RuBP and the fun ctioning forms of the enzyme in chloroplasts could be predicted using the kinetic data of the binding.