A SENSITIVE ELISA FOR 6-BETA-HYDROXYCORTISOL IN URINE USING ENZYME PENICILLINASE (BETA-LACTAMASE)

Authors
PATEL SB KHATKHATAY I DESAI MP BETRABET SS TODDYWALLA VS
Citation
Sb. Patel et al., A SENSITIVE ELISA FOR 6-BETA-HYDROXYCORTISOL IN URINE USING ENZYME PENICILLINASE (BETA-LACTAMASE), Journal of steroid biochemistry and molecular biology, 48(2-3), 1994, pp. 293-296
Citations number
17
Categorie Soggetti
Biology,"Endocrynology & Metabolism
Journal title
Journal of steroid biochemistry and molecular biology → ACNP
ISSN journal
09600760
Volume
48
Issue
2-3
Year of publication
1994
Pages
293 - 296
Database
ISI
SICI code
0960-0760(1994)48:2-3<293:ASEF6I>2.0.ZU;2-O
Abstract
A sensitive and specific, enzyme labelled immunosorbent assay (ELISA) for 6beta-hydroxycortisol in diluted urine using penicillinase was dev eloped. 6beta-Hydroxycortisol-21-hemisuccinate was conjugated with enz yme penicillinase. Antibody immobilized on a polyvinylchloride ELISA p late (Dynatech) was used for separation of bound from free ligand. The sensitivity of the assay was between 2.0-3.0 pg per well and recovery of 6beta-hydroxycortisol from urine ranged between 85.0-108.0%. The a ssay is simple, rapid and precise.