S. Devi et al., AN IMPROVED METHOD FOR RAPID FIXATION AND EMBEDDING OF PTERIDOPHYTE PLANT MATERIALS, Biotechnic & histochemistry, 69(2), 1994, pp. 99-101
A rapid method for fixation and embedding of plant materials, especial
ly pteridophytes, is suggested. Addition of tannic acid following osmi
cation improved the visualization of membranes. Staining en bloc with
uranyl acetate between osmication and tannic acid is suggested for tis
sues infected with fungi and bacteria.