Cc. Yu et al., RADIOLABELING LEUKOCYTES WITH TC-99(M)-STANNOUS CHLORIDE COLLOID FOR THE DETECTION OF INFLAMMATORY LESIONS, Nuclear medicine communications, 18(1), 1997, pp. 70-74
Freshly prepared Tc-99(m)-stannous chloride colloid (Tc-99(m)-SCC) was
used to label human leukocytes. The radiolabelled leukocytes were the
n injected intravenously into rats bearing carrageenan-induced inflamm
ation in their hindlimbs. Scintigraphic imaging (n = 3) and biodistrib
ution studies (n = 4) 4 h post-injection were performed. The ratio of
radioactivity (cpm/pixel) accumulated in the inflammation site to that
in the corresponding area of the contralateral limb was 2.9 +/- 0.8,
as calculated by region-of-interest analyses of the planar images. The
distribution of radioactivity in the dissected tissue showed that the
ratio of radioactivity (cpm/g) of infected tissue to that of a normal
sample obtained from the contralateral Limb was 2.6 +/- 0.5. The biod
istribution study of dissected organs showed high uptake in liver (58.
9 +/- 8.2%), spleen (4.3 +/- 1.8%) and lungs (6.3 +/- 2.4%). The resul
ts of this preliminary study indicate that Tc-99(m)-SCC can be used as
an alternative agent for radiolabelling leukocytes for the detection
of inflammatory lesions.