CLONING OF A LOCUS INVOLVED IN PATHOGENICITY AND PRODUCTION OF EXTRACELLULAR POLYSACCHARIDE AND PROTEASE IN XANTHOMONAS-CAMPESTRIS PV CAMPESTRIS

A Tn5-induced mutant of Xanthomonas campestris pv campestris deficient in protease and extracellular polysaccharide production, and non-path ogenic to turnip seedlings, was isolated. Recombinant clones which res tored all three characteristics concomitantly were obtained from a wil d-type genomic library. One of the plasmid clones, pIJ3090, showed no hybridization to previously isolated clones involved In protease produ ction. The mutant was not also complemented by other known clones invo lved in pathogenicity, in xanthan gum production or in protease produc tion.