TRANSFER AND EXPRESSION OF THE CRYIVB AND CRYIVD GENES OF BACILLUS-THURINGIENSIS SUBSP ISRAELENSIS IN BACILLUS-SPHAERICUS-2297

Authors
PONCET S DELECLUSE A ANELLO G KLIER A RAPOPORT G
Citation
S. Poncet et al., TRANSFER AND EXPRESSION OF THE CRYIVB AND CRYIVD GENES OF BACILLUS-THURINGIENSIS SUBSP ISRAELENSIS IN BACILLUS-SPHAERICUS-2297, FEMS microbiology letters, 117(1), 1994, pp. 91-95
Citations number
11
Categorie Soggetti
Microbiology
Journal title
FEMS microbiology letters → ACNP
ISSN journal
03781097
Volume
117
Issue
1
Year of publication
1994
Pages
91 - 95
Database
ISI
SICI code
0378-1097(1994)117:1<91:TAEOTC>2.0.ZU;2-2
Abstract
The genes encoding the CryIVB and CryIVD crystal polypeptides of B. th uringiensis subsp. israelensis were cloned independently on a stable s huttle vector, and transferred into B. sphaericus 2297. Recombinant ce lls expressed the B. thuringiensis toxins during sporulation and were shown to be toxic to Aedes aegypti fourth instar larvae, whereas the p arental strain was not.