A. Okamoto et al., REGENERATION OF PLANTLETS FROM CELERY (APIUM-GRAVEOLENS L) CALLUS USING A FERMENTER, Journal of fermentation and bioengineering, 77(2), 1994, pp. 208-211
The efficiency of somatic embryogenesis of calluses induced from celer
y (Apium graveolens L.) (Fl variety, 1026-2) in an air-lift type ferme
ntor was about half of that in flasks. The efficiency of somatic embry
o maturation in an air-lift type fermentor was almost the same as that
in flasks, and the efficiency in a stirred-tank type fermentor was ab
out 70% of that in an air-lift type. We succeeded in producing 1,590,0
00 somatic embryos, and 296,000 plantlets after maturation by batch cu
lture using a 30-l air-lift type fermentor.