J. Sandhu et al., HUMAN PRIMARY IMMUNE-RESPONSE IN SCID MICE ENGRAFTED WITH HUMAN PERIPHERAL-BLOOD LYMPHOCYTES, The Journal of immunology, 152(8), 1994, pp. 3806-3813
Severe combined immune deficient (SCID) mice were engrafted with human
peripheral blood lymphocytes (Hu-PBLs) after treatment with anti-asia
lo GM-1 antiserum and radiation. This pretreatment facilitates high le
vel of Hu-PBL engraftment in the SCID mice spleen. The next day, the H
u-PBL-SCID mice were immunized with either keyhole limpet hemocyanin (
KLH), or carbohydrate Ag STn (AcNeu-alpha 2-alpha 6-Gal NAc-0) conjuga
ted to KLH or protein of circumsporozoite malaria parasite (CSP), in a
mixture of complete and incomplete Freund's adjuvant (1:10 v/v). The
mice were bled 14 to 16 days after immunization, and the sera analyzed
for STn-, KLH-, and CSP-specific human Ige and IgM Abs. The results s
howed that the immunized animals had a significantly higher titer of A
g-specific human Ige and IgM Abs compared to the control Hu-PBL-SCID m
ice. No significant Ab cross-reactions were detected between sera from
KLH-, STn-, and CSP-vaccinated Hu-PBL-SCID mice. Depletion of either
human CD4(+) or CD8(+) cells, in Hu-PBL-SCID mice, showed that CD4(+)
cells were essential for the primary immune response, but depletion of
CD8(+) cells had no influence on the titer of Ag-specific human Ige a
nd IgM Abs.