PERIPHERAL-NERVE STIMULATION INCREASES SEROTONIN AND DOPAMINE METABOLITES IN RAT SPINAL-CORD

Extracellular serotonin (5-HT), dopamine (DA), and their metabolites, 5-hydroxyindoleacetic acid (5-HIAA), 3,4-dihydroxyphenylacetic acid (D OPAC), and homovanillic acid (HVA), were assessed in the rat lumbar sp inal cord (L3-4) by in vivo microdialysis with high performance liquid chromatography and electrochemical detection (HPLC-ECD). Under uretha ne-chloralose anesthesia, basal levels of 5-HT and DA in the dialysate s were approximately 1.0-1.2 pg/22 mu l sample, 5-HIAA, DOPAC, and HVA were constant at 322.6 +/- 14.9, 8.6 +/- 0.7, and 10.4 +/- 0.4 pg/22 mu l sample (mean +/- SE), respectively. Local application of 100 mM K Cl via the dialysis probe increased the 5-HT and DA. Peripheral nerve stimulation that selectively excited the large (A-beta) or small (A-de lta) myelinated fibers increased the metabolites. Excitation of the A- beta fibers increased the levels of 5-HIAA to 138%, DOPAC to 155%, and HVA to 143% of the controls. Stimulation of the A-delta fibers increa sed 5-HIAA to 121%, DOPAC to 120%, and HVA to 124% of the controls. Th e results suggest that nonnociceptive peripheral nerve stimulation may activate the descending 5-HT and DA systems in the spinal cord.