This paper describes preparation of the isolated brain of hibernating
ground squirrel maintained by intraarterial perfusion. This technique
allows a long-term survival (about 3 days) of the isolated brain of ad
ult animals. The preparation viability was assessed by extracellular i
nvestigation of stability of structure-specific electrical activity. T
he areas investigated include neocortex, hippocampus, thalamus, hypoth
alamus, and brain stem.