A simple test for diagnosing Nosema apis infections in honey bees, whi
ch does not require a microscope, is described. Bee abdomens are crush
ed in water in plastic 1.5 ml microcentrifuge tubes, nigrosin stain is
added and the tube left to stand vertically overnight. Centrifugation
of the tubes eliminates the need for an overnight wait. The nigrosin
stains any bee tissues and most pollen a dark blue/black and the N. ap
is spores settle to form a pale grey, unstained pellet at the bottom o
f the tube. Eight million or more spores per bee can be detected (P <
0.05) and the size of the pellet gives an indication of the magnitude
of the spore load. Pooled samples of bees crushed in a plastic bag may
be similarly assessed, although some accuracy is lost.