FACULTATIVE EXPRESSION OF HYGIENIC BEHAVIOR OF HONEY-BEES IN RELATIONTO DISEASE RESISTANCE

Four experiments were conducted to examine factors that influence the expression of hygienic and non-hygienic behaviour in honey bees, Apis mellifera, and to examine the correlation between this behaviour and r esistance to chalkbrood, Ascosphaera apis. Colonies were headed by ins trumentally inseminated queens selected on the basis of uncapping and removal behaviour expressed by their progeny. In the first experiment, colony strength was altered by transferring hygienic and non-hygienic colonies from 10-frame field hives to 2-frame observation hives. This treatment significantly reduced the hygienic response of the hygienic bees but did not affect the response of the non-hygienic bees. In the second experiment, hygienic and non-hygienic bees displayed different responses to freeze-killed and live brood which had been partially or entirely uncapped. Both lines of bees recapped both partially and ent irely uncapped live brood, but non-hygienic bees also recapped partial ly uncapped freeze-killed brood, suggesting that non-hygienic bees eit her could not detect dead or diseased brood or avoided it by sealing i t within a comb cell. The third experiment tested whether the degree o f hygienic behaviour could be increased by adding hygienic bees to non -hygienic colonies. Adding 20-30% young hygienic bees to non-hygienic colonies did not increase the degree of hygienic behaviour, but adding young non-hygienic bees to hygienic colonies suppressed the behaviour . The results suggest that although hygienic behavior is genetically d etermined, its expression depends on colony strength and composition o f workers within the colony. In the fourth experiment, the hygienic an d non-hygienic colonies were fed with pollen patties containing A. api s spores. The weak correspondence that was observed between removal be haviour and physiological resistance to chalkbrood suggested that few colonies are both highly hygienic and physiologically resistant to cha lkbrood. Selection against uncapping and removing diseased brood might occur if this behaviour also promotes the spread of disease through t he colony. This possibility is discussed in relation to avoidance beha viour of other social insects toward pathogens.