Three ammonium halide type surfactants were tested under standard labo
ratory condition for biodegradability and acute toxicity to Daphnia ma
gna and in the Microtox test with the luminescent bacterium Photobacte
rium phosphoreum. The tested compounds are dimethyldistearylammonium c
hloride (DSDMAC), cetyltrimethylammonium bromide (HTMAB) and cetylbenz
yldimethylammonium chloride (HBDMAC). The results indicate DSDMAC is l
ess toxic than the other two surfactants and that it biodegrades rapid
ly at both lower (2.5 mg/L) and higher (10 mg/L) concentrations.