A. Colbeau et al., CLONING AND SEQUENCES OF THE STRUCTURAL (HUPSLC) AND ACCESSORY (HUPDHI) GENES FOR HYDROGENASE BIOSYNTHESIS IN THIOCAPSA-ROSEOPERSICINA, Gene, 140(1), 1994, pp. 25-31
The first molecular biology study on the purple sulfur photosynthetic
bacterium Thiocapsa roseopersicina is reported, namely, the constructi
on of cosmid libraries and isolation of a hydrogenase gene cluster by
hybridization with hydrogenase structural genes from the purple non-su
lfur bacterium, Rhodobacter capsulatus. The sequenced gene cluster con
tains six open reading frames, the products of which show significant
degrees of identity (from 40 to 78%) with hydrogenase gene products ne
cessary for biosynthesis of the group-I of [NiFe]hydrogenases. The str
uctural hupSLC genes encode the small and large hydrogenase subunits a
nd a hydrophobic protein shown to accept electrons from hydrogenase in
R. capsulatus. They are followed downstream by three genes, hupDHI, w
hich are similar to hydrogenase accessory genes found in other bacteri
a.