A RELA((S)) SUPPRESSOR MUTANT ALLELE OF BACILLUS-SUBTILIS WHICH MAPS TO RELA AND RESPONDS ONLY TO CARBON LIMITATION

Authors
GROPP M EIZENMAN E GLASER G SAMARRAI W RUDNER R
Citation
M. Gropp et al., A RELA((S)) SUPPRESSOR MUTANT ALLELE OF BACILLUS-SUBTILIS WHICH MAPS TO RELA AND RESPONDS ONLY TO CARBON LIMITATION, Gene, 140(1), 1994, pp. 91-96
Citations number
16
Categorie Soggetti
Genetics & Heredity
Journal title
Gene → ACNP
ISSN journal
03781119
Volume
140
Issue
1
Year of publication
1994
Pages
91 - 96
Database
ISI
SICI code
0378-1119(1994)140:1<91:ARSMAO>2.0.ZU;2-L
Abstract
The histidine analog 3-amino-1,2,4-triazole (AT) was used for the sele ction of spontaneous AT-resistant revertants of a relA mutant of Bacil lus subtilis. One of these revertants, L3, showed a unique phenotype; it did not respond to amino acid starvation, like the relA mutant, but it did respond to glucose starvation by the accumulation of (p)ppGpp, unlike its parent. Genetic analysis revealed that this suppressor mut ant (relA((S))) allele mapped to the relA locus at 239 degrees on the B. subtilis chromosome.