EFFECT OF GROWTH-RATE ON THE EICOSAPENTAENOIC ACID AND DOCOSAHEXAENOIC ACID CONTENT OF ISOCHRYSIS-GALBANA IN CHEMOSTAT CULTURE

Authors
GRIMA EM PEREZ JAS CAMACHO FG SEVILLA JMF FERNANDEZ FGA
Citation
Em. Grima et al., EFFECT OF GROWTH-RATE ON THE EICOSAPENTAENOIC ACID AND DOCOSAHEXAENOIC ACID CONTENT OF ISOCHRYSIS-GALBANA IN CHEMOSTAT CULTURE, Applied microbiology and biotechnology, 41(1), 1994, pp. 23-27
Citations number
15
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Applied microbiology and biotechnology → ACNP
ISSN journal
01757598
Volume
41
Issue
1
Year of publication
1994
Pages
23 - 27
Database
ISI
SICI code
0175-7598(1994)41:1<23:EOGOTE>2.0.ZU;2-7
Abstract
An isolate of Isochrysis galbana rich in eicosapentaenoic acid (EPA) a nd docosahexaenoic acid (DHA) has been grown as a chemostat culture at 20 degrees C and pH 8.00 controlled by CO2 injection. From a low dilu tion rate (D) of 0.0024 h(-1) to 0.0377 h(-1), close to maximum growth , a decrease in EPA content from 5.21% dry weight (d.w.) to 2.80% d.w. was observed, although the percentage of EPA in the total fatty acids increased. Lipids were fractionated, EPA being the major fatty acid f ound in the glycolipid fraction, whereas in the neutral lipid fraction were mainly palmitic and palmitoleic acids. At the same time, the bio mass concentration also decreased from 1015 mg.l(-1) to 202 mg.l(-1) o ver the range of Ds mentioned. Nonetheless, EPA. productivity had a ma ximum value of 15.26 mg.l(-1) day(-1) at D = 0.0208 h(-1).