PRODUCTION OF HORMOCONIS-RESINAE GLUCOAMYLASE-P BY A STABLE INDUSTRIAL STRAIN OF SACCHAROMYCES-CEREVISIA

Authors
VAINIO AEI LANTTO R PARKKINEN EEM TORKKELI HT
Citation
Aei. Vainio et al., PRODUCTION OF HORMOCONIS-RESINAE GLUCOAMYLASE-P BY A STABLE INDUSTRIAL STRAIN OF SACCHAROMYCES-CEREVISIA, Applied microbiology and biotechnology, 41(1), 1994, pp. 53-57
Citations number
22
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Applied microbiology and biotechnology → ACNP
ISSN journal
01757598
Volume
41
Issue
1
Year of publication
1994
Pages
53 - 57
Database
ISI
SICI code
0175-7598(1994)41:1<53:POHGBA>2.0.ZU;2-3
Abstract
A stable strain of Saccharomyces cerevisiae secreting glucoamylase (EC 3.2.1.3) with high debranching activity was constructed using recombi nant DNA technology. An expression cassette without bacterial sequence s, containing Hormoconis resinae glucoamylase P cDNA and the dominant selection marker MEL1 was integrated into the yeast chromosome using A RS1 homology. The glucoamylase expression level of the integrant yeast strain was increased by chemical mutagenesis. The yeast strains secre ting glucoamylase were able to grow on soluble starch (5%, w/v) and fe rment it to ethanol.