P. Caumette et al., MICROBIAL MATS IN THE HYPERSALINE PONDS OF MEDITERRANEAN SALTERNS (SALINS-DE-GIRAUD, FRANCE), FEMS microbiology, ecology, 13(4), 1994, pp. 273-286
Microbial mats that develop in the gypsum crust of the hypersaline pon
ds of Salins-de-Giraud (Camargue, France) were carefully investigated
between 1989 and 1991. During the warm seasons, when these mats were f
ully developed, analyses of microbial activities and microprofiles of
oxygen and sulfide have shown a great activity of the different kinds
of bacteria found in the mat below the gypsum crust. Oxygen production
could amount to 2 mu mol cm(-3) h(-1) during the maximum daylight whe
reas the oxidation of sulfide in the light was calculated to be 12.7 m
u mol cm(-3) h(-1), i.e. 300 to 180 mmol m(-2) day-l assuming 8-10 hou
rs of constant daylight and a sulfide oxidation zone of 3 mm in thickn
ess. This sulfide oxidation consumes about 65-95% of the diel sulfide
production which has been estimated to be 400 to 450 mmol m(-2) day-l
originating from sulfate reduction which takes place in the 6 cm depth
horizon of sediment plus mat. According to the amounts of sulfate pre
cipitated at the sediment surface in the form of gypsum, sulfate reduc
tion is never limited and was found to be among the highest values rep
orted in the literature (average value of 8200 nmols cm(-3) day(-1)).
Completely covered by the gypsum crust, this ecosystem has been found
to react as a closed system. Consequently, the sulfide does not escape
and accumulate below the crust. It was detected up to the top of the
mat after a few hours of darkness. It is reoxidized during the day by
the photosynthetic organisms that form the mats. These latter mats wer
e composed of 2 to 3 laminated layers of phototrophic organisms: an up
per brown layer of the cyanobacterium Aphanothece, an intermediate gre
en layer of the cyanobacterium Phormidium and an underlying red layer
of purple sulfur-oxidizing bacteria from which two new halophilic spec
ies were isolated (Chromatium salexigens and Thiocapsa halophila). It
has been found that the accumulated sulfide is oxidized not only by th
e phototrophic bacteria in the sulfide oxidation zone but also by the
oxygen produced by the cyanobacteria which are able to photosynthesize
in the presence of sulfide.