Objective: To determine the frequency of glove contamination associate
d with fetal extraction during cesarean delivery. Methods: The study w
as performed in 25 women having scheduled or unscheduled cesarean deli
very. Surgeons double-gloved for all procedures. Immediately before an
d after delivery of the fetus, the dorsal aspect of the fingers an han
d of the surgeon's outer glove was swabbed with cotton tip applicators
and cultured for aerobic and anaerobic organ isms. Only the glove fro
m the hand that was used to deliver the infant was cultured. Results:
Nine of 25 cultures (36%, 95% confidence interval [CI] 17-55) performe
d immediately before fetal extraction were positive for staphylococci.
No other organisms were isolated. Cultures performed following fetal
extraction showed non-staphylococcal bacteria in 11 of 14 (79%, 95% CI
58-100) laboring women and one of 11 (9%, 95% CI 0-26) nonlaboring wo
men, a statistically significant difference (P < .01). In the laboring
patients, non-staphylococcal bacteria were isolated with similar freq
uency from the dorsal aspect of the hand (seven of 14, 50%, 95% CI 24-
76) and the fingers (ten of 14, 71%, 95% CI 47-95). These cultures yie
lded mostly bacterial species from the Enterobacteriaceae family. Conc
lusion: In laboring patients with ruptured membranes, delivery of the
fetal head frequently results in contamination of the surgeon's glove
with pathogenic bacteria. This finding may partially explain the incre
ased frequency of post-cesarean endometritis associated with manual ex
traction of the placenta.