2 TYROSINASE NONAPEPTIDES RECOGNIZED ON HLA-A2 MELANOMAS BY AUTOLOGOUS CYTOLYTIC T-LYMPHOCYTES

A number of cytolytic T lymphocyte (CTL) clones derived from several m elanoma patients have been found to recognize a majority of melanomas from HLA-A2 patients. We have reported previously that two such CTL cl ones recognize a product of the tyrosinase gene that is presented by H LA-A2. Here we show that one of these CTL clones recognizes a peptide encoded by the first nine amino acids of the putative signal sequence of tyrosinase. The other CTL clone recognizes a different tyrosinase p eptide corresponding to amino acids 368-376. Both peptides contain con sensus motifs of HLA-A2 binding peptides.