ISOENZYMATIC VARIABILITY OF COLLETOTRICHU M-LINDEMUTHIANUM ISOLATES WITH DIFFERENT VIRULENCE LEVEL

A methodology for extraction and isoenzyme analysis of monoconidial is olates of Colletotrichum lindemutbianum belonging to different physiol ogical races was developed as an additional criterium to characterize races and study the genetics of the pathogen. Among the enzymes studie d on starch gel electrophoresis, ALDH, ADH, ALD, ME, alpha-EST, beta-E ST, ACP, PGI, HK, MDH and KADH displayed interpretable resolution and activity. Twenty-two isoenzymatic loci were detected from which 13 wer e polymorphic, and considered as potential markers to study the geneti c variability in C. lindemuthianum. The relatively large number of phe notypic/genotypic categories reflected the occurrence of a broad heter ogeneity in the sampled population. The race grouping based on isoenzy me analysis did not completely agree with those published on the basis of the response to inoculation on host plant differentials.