M. Tshamala et al., BIOMECHANICAL PROPERTIES OF ETHYLENE-OXIDE STERILIZED AND CRYOPRESERVED CORTICAL BONE ALLOGRAFTS, Veterinary and comparative orthopaedics and traumatology, 7(1), 1994, pp. 25-30
To evaluate changes during preservation, 76 femoral segments of cortic
al bone of dogs were tested biomechanically in order to determine thei
r strength in compression, in bending and in torsion. These bones were
wrapped in plastic-paper and they were sterilized with ethylene oxide
12%, at 30-degrees-C and 1.4 bars and preserved thereafter at -20-deg
rees-C. According to the applied loading test, three treatment groups
were devised; each treatment group was subdivided into five groups: on
e control (fresh specimen) and four test-groups differing from each ot
her by the preservation time; i.e. 3, 6, 9 or 12 months. Statistical a
nalysis has demonstrated that there is not a significant difference be
tween the test results for each test-group and its control. The conclu
sion of this study was that bones, treated as described above, can be
stored up to one year without risks to significantly reduce their stre
ngth in compression, in bending and in torsion.