THE INDUCTION OF MURINE TUMOR-INFILTRATING LYMPHOCYTES (TIL) BY INTERLEUKIN-2 OR T-CELL GROWTH-FACTOR

Mice were injected in the foot pad with either 5x10(5) syngeneic plasm acytoma (MOPC104E) or fibrosarcoma cells (Meth A). Lymph nodes contain ing tumor cells were harvested 14 days later and cultured. In the pres ence of recombinant interleukin-2 (r-IL-2) predominantly tumor cells p roliferated. Culture with T cell growth factor (TCGF) resulted in the growth of lymphoid cells. Concanavalin A (Con A) had only a modest eff ect on elimination of tumor cells in the culture. Tumor-infiltrating l ymphocytes (TIL) prepared from the lymph nodes showed specific tumor-n eutralizing activity when grown in the presence of TCGF. In vitro exam ination revealed that Meth A cells could not be lysed by TIL, while TI L from MOPC tumors showed tumor specific activity. This study may expl ain negative results in human trials with TIL induced by IL-2 alone.