THE P2Y(1) RECEPTOR IS AN ADP RECEPTOR ANTAGONIZED BY ATP AND EXPRESSED IN PLATELETS AND MEGAKARYOBLASTIC CELLS

The human P2Y(1) purinoceptor has been expressed in Jurkat cells and t he effects of HPLC purified nucleotides on calcium movements were meas ured. The most potent agonist was 2-methylthio-ADP followed by ADP, AT P, Sp-ATP alpha S and B,gamma-methylene-ATP were competitive antagonis ts, Suramin and PPADS inhibited the effects of ADP, This pharmacologic al profile is tbe same as that of the so-called P2T purinoceptor respo nsible for platelet aggregation, which has not yet been identified, Us ing PCR we found the P2Y(1) receptor to be present in blood platelets and megakaryoblastic cell lines. These data suggest that the P2Y(1) re ceptor may be the elusive P2T receptor. (C) 1997 Federation of Europea n Biochemical Societies.