A REVERSED-PHASE HPLC METHOD FOR THE SIMULTANEOUS DETERMINATION OF ALL MONOSACCHARIDES CONTAINED IN GALACTOSAMINOGLYCAN ISOMERS FROM HUMAN AORTA PROTEOGLYCANS

Authors
FORMATO M SENES A SOCCOLINI F COINU R CHERCHI GM
Citation
M. Formato et al., A REVERSED-PHASE HPLC METHOD FOR THE SIMULTANEOUS DETERMINATION OF ALL MONOSACCHARIDES CONTAINED IN GALACTOSAMINOGLYCAN ISOMERS FROM HUMAN AORTA PROTEOGLYCANS, Carbohydrate research, 255, 1994, pp. 27-39
Citations number
32
Categorie Soggetti
Chemistry Inorganic & Nuclear
Journal title
Carbohydrate research → ACNP
ISSN journal
00086215
Volume
255
Year of publication
1994
Pages
27 - 39
Database
ISI
SICI code
0008-6215(1994)255:<27:ARHMFT>2.0.ZU;2-8
Abstract
Monosaccharides obtained by reduction and hydrolysis of galactosaminog lycan isomers, are entirely determined as their perbenzoyl derivatives by reversed phase HPLC, without removal of hexosamines prior to benzo ylation. The method is suitable for the analysis of arterial proteogly can constituent galactosaminoglycans, providing specific, precise and. reproducible results. Moreover, synthesis and characterization of tri -O-benzoyl-1,6-L-anhydroidose and N-benzoyl-tetra-O-benzoyl-alpha- and -beta-D-galactosamine have been accomplished