CLONING OF A PARATHYROID HORMONE PARATHYROID HORMONE-RELATED PEPTIDE RECEPTOR (PTHR) CDNA FROM A RAT OSTEOSARCOMA (UMR-106) CELL-LINE - CHROMOSOMAL ASSIGNMENT OF THE GENE IN THE HUMAN, MOUSE, AND RAT GENOMES/

Complementary DNAs spanning the entire coding region of the rat parath yroid hormone/parathyroid hermone-related peptide receptor (PTHR) were isolated from a rat osteosarcoma (UMR 106) cell-line cDNA iibrary. Th e longest of these clones (rPTHrec4) was used to chromosomally assign the PTHR gene in the human, rat, and mouse genomes. By somatic cell hy brid analysis, the gene was localized to human chromosome 3 and rat ch romosome 8; by in situ hybridization, the gene was mapped to human chr omosome 3p21.1-p22 and to mouse chromosome 9 band F; and by interspeci fic backcross analysis, the Pthr gene segregated with the transferrin (Trf) gene in chromosome 9 band F. Mouse chromosome 9 and rat chromoso me 8 are known to be highly homologous and to also show synteny conser vation with human chromosome 3. These three chromosomes share the tran sferrin gene (TF), the myosin light polypeptide 3 gene (MYL3), and the acylpeptide hydrolase gene (APEH). Our results add a fourth gene, the PTHR gene, to the synteny group conserved in these chromosomes. (C) 1 994 Academic Press, Inc.