PHYSICAL MAPPING OF CHROMOSOME-17 COSMIDS BY FLUORESCENCE IN-SITU HYBRIDIZATION AND DIGITAL IMAGE-ANALYSIS

We used fluorescence in situ hybridization and digital image analysis to localize cosmids along human chromosome 17. Seventy-one cosmids wer e selected at random from a chromosome 17 library constructed from a p artial Sau3AI digest of flow-sorted chromosomes from a mouse-human hyb rid cell line. Sixty-three of these (89%) gave a signal only on chromo some 17. The 40 cosmids producing the most distinct hybridization sign als in metaphase and interphase cells were precisely mapped using digi tal image analysis. An additional 20 cosmids, previously mapped by lin kage analysis, were also mapped. The order of these probes determined by metaphase mapping was consistent with the order determined by linka ge analysis. (C) 1994 Academic Press, Inc.