PLATELETS DO NOT MODULATE LEUKOCYTE-MEDIATED CORONARY MICROVASCULAR DAMAGE DURING EARLY REPERFUSION

Several studies indicate that leukocytes and platelets exacerbate the compromise of myocardial function that occurs after ischemia-reperfusi on (I/R). However, it is unclear whether both leukocytes and platelets must be present to mediate coronary microvascular damage early during reperfusion after ischemia. To examine the effects of leukocytes and platelets on microvascular damage after I/R, we measured transcoronary albumin extravasation (O/I), perfused coronary capillary density (Cap s), and transcoronary albumin extravasation per perfused capillary [(O /I)/Caps] in isolated rat hearts perfused with a Krebs-albumin-red blo od cell solution [K(S)RBC], whole rat blood diluted with Krebs buffer (DWB), leukocyte-free, platelet-rich DWB (LFB), or leukocyte-rich, pla telet-free DWB (LRB) before and after a 30-min period of global, no-fl ow ischemia. We found that in isolated hearts perfused with K(B)RBC be fore ischemia, O/I values were significantly increased (+68%, P < 0.01 ) and Caps values were significantly decreased (-25%, P < 0.05) after 25 min of reperfusion. A similar pattern of O/I values (+72%, P < 0.01 ) and Caps values (-40%, P < 0.05) was observed in hearts perfused wit h LFB. These effects were exacerbated in hearts perfused with DWB or L RB. O/I values were increased 90% (P < 0.01), and Caps values were dec reased 62% (P < 0.01) in the DWB-perfused hearts. Similar increases in O/I values (+82%, P < 0.01) and decreases in Caps values (-65%, P < 0 .01) were measured in the LRB-perfused hearts. Additionally, (O/I)/Cap s values were significantly increased in the hearts perfused with DWB (+93%, P < 0.01) and LRB (+84%, P < 0.01) compared with the hearts per fused with K(2)RBC or LFB. These results suggest that interactions bet ween leukocytes and platelets are not requisite for the development of coronary microvascular damage early during reperfusion after ischemia .