A THIOREDOXIN FROM THE EXTREME THERMOPHILIC ARCHAEON SULFOLOBUS-SOLFATARICUS

Authors
GUAGLIARDI A NOBILE V BARTOLUCCI S ROSSI M
Citation
A. Guagliardi et al., A THIOREDOXIN FROM THE EXTREME THERMOPHILIC ARCHAEON SULFOLOBUS-SOLFATARICUS, International Journal of Biochemistry, 26(3), 1994, pp. 375-380
Citations number
33
Categorie Soggetti
Biology
Journal title
International Journal of Biochemistry → ACNP
ISSN journal
0020711X
Volume
26
Issue
3
Year of publication
1994
Pages
375 - 380
Database
ISI
SICI code
0020-711X(1994)26:3<375:ATFTET>2.0.ZU;2-J
Abstract
1. A purification procedure for a thioredoxin from the extremophilic a rchaeon Sulfolobus solfataricus is described. 2. The thioredoxin is ac tive in the dithiothreitol-dependent reduction of insulin disulfide bo nds. 3. The thioredoxin is a monomer of 24,800 Da; it is an acidic pro tein with a pI of 4.5. 4. The protein is stable to heating for 3 hr at 90-degrees-C. 5. The amino acid composition of S. solfataricus thiore doxin is reported.