Rm. Puttick et al., GALANIN-INDUCED MEMBRANE DEPOLARIZATION OF NEONATAL RAT CULTURED DORSAL-ROOT GANGLION-CELLS, European journal of pharmacology, 254(3), 1994, pp. 303-306
In this study we examined the effects of galanin on membrane currents
recorded from neonatal rat cultured dorsal root ganglion neurones usin
g the whole-cell patch-clamp technique. When neurones were voltage-cla
mped at -60 mV, galanin (1-300 nM) evoked an inward current associated
with an increase in input conductance. These effects were observed in
21 of the 33 dorsal root ganglion neurones studied and were not atten
uated by the galanin receptor antagonist galantide. Galanin fragments,
galanin-(1-16) and galanin-(21-29) (300 nM) also elicited an inward c
urrent at -60 mV. The inward current induced by galanin was not linear
ly related to membrane potential indicating that the membrane current
response was the result of more than one ionic event.