DETERMINATION OF BOLDINE IN DRUG FORMULATIONS BY 1ST-DERIVATIVE SYNCHRONOUS SPECTROFLUOROMETRY

A simple and sensitive method is proposed for the determination of bol dine by first-derivative synchronous spectrofluorimetry based on its n ative fluorescence in 0.1 N sulphuric acid. The constant wavelength di fference (DELTAlambda = lambda(cm) - lambda(ex)) chosen to optimize th e determination is 90 nm. The linear concentration range of applicatio n is between 2.0 and 50.0 mug l-1 of boldine, the detection limit 0.4 mug l-1 and the relative standard deviation at a concentration of 25 m ug l-1 was 2.1%. The method has been satisfactorily applied to the det ermination of boldine in pharmaceutical formulations.