DEVELOPMENT OF AN RNA RNA HYBRIDIZATION ASSAY FOR THE DETECTION OF THE HAV CF53 STRAIN/

Authors
VANCUYCKGANDRE H LIVACHE T RINGEAUD J CRANCE JM
Citation
H. Vancuyckgandre et al., DEVELOPMENT OF AN RNA RNA HYBRIDIZATION ASSAY FOR THE DETECTION OF THE HAV CF53 STRAIN/, Research in virology, 145(1), 1994, pp. 37-43
Citations number
32
Categorie Soggetti
Virology
Journal title
Research in virology → ACNP
ISSN journal
09232516
Volume
145
Issue
1
Year of publication
1994
Pages
37 - 43
Database
ISI
SICI code
0923-2516(1994)145:1<37:DOARRH>2.0.ZU;2-T
Abstract
A quick and sensitive dot-blot assay using non-radioactive labelled RN A probes was developed for the detection of the CF53 strain of hepatit is A virus (HAV) in cell culture. The cDNA of the 5' end of the HM175 strain was inserted in a transcription vector pSPT18 and was used to s ynthesize P-32- or digoxigenin-labelled RNA probes. These RNA probes s pecifically detected the RNA of the CF53 strain and can be used to det ect HAV in PLC/PRF/5 cells. The sensitivity of non-radioactive tests w as comparable to that of radiolabelled probes.