PRESERVATION OF PROTEIN PHOSPHORYL GROUPS IN IMMUNOPRECIPITATION ASSAYS

Authors
PAPAVASSILIOU AG
Citation
Ag. Papavassiliou, PRESERVATION OF PROTEIN PHOSPHORYL GROUPS IN IMMUNOPRECIPITATION ASSAYS, Journal of immunological methods, 170(1), 1994, pp. 67-73
Citations number
20
Categorie Soggetti
Immunology
Journal title
Journal of immunological methods → ACNP
ISSN journal
00221759
Volume
170
Issue
1
Year of publication
1994
Pages
67 - 73
Database
ISI
SICI code
0022-1759(1994)170:1<67:POPPGI>2.0.ZU;2-Z
Abstract
A phosphorylation-sensitive immunoprecipitation protocol is presented which ensures quantitative and qualitative recovery of phosphoantigens from different phosphatase environments. The protocol is based on the phosphatase-inactivating properties of a specific mixture of chemical compounds included in the ''classical'' immunoprecipitation buffers.