EXPRESSION OF P47-PHOX AND P67-PHOX PROTEINS IN MURINE BONE-MARROW-DERIVED MACROPHAGES - ENHANCEMENT BY LIPOPOLYSACCHARIDE AND TUMOR-NECROSIS-FACTOR-ALPHA BUT NOT COLONY-STIMULATING FACTOR-1

We have investigated the relationship between the expression of the p4 7-phox and p67-phox cytosolic components of the NADPH oxidase and prim ing of the macrophage respiratory burst. Western blot analysis reveale d that murine bone marrow-derived macrophages (BMM) contain immunoreac tive proteins detected by antisera raised against recombinant human p4 7-phox and p67-phox. Priming BMM by exposure to tumor necrosis factor alpha (TNF-alpha) or lipopolysaccharide (LPS) increased the levels of p47-phox and p67-phox. Colony-stimulating factor 1 (CSF-1), which we p reviously found to have a negative effect on the priming of murine mac rophages, had no effect on the level of p47-phox but down-regulated th at of p67-phox. Our results suggest that the regulatory effects of LPS , TNF-alpha, and CSF-1 on the respiratory burst of BMM may be due to m odulation of the expression of the p47-phox and p67-phox cytosolic com ponents of the NADPH oxidase.