CRYSTALLIZATION AND PRELIMINARY-X-RAY ANALYSIS OF A LIPASE FROM CHROMOBACTERIUM-VISCOSUM

Authors
LANG D HAALCK L HOFMANN B HECHT HJ SPENER F SCHMID RD SCHOMBURG D
Citation
D. Lang et al., CRYSTALLIZATION AND PRELIMINARY-X-RAY ANALYSIS OF A LIPASE FROM CHROMOBACTERIUM-VISCOSUM, Acta crystallographica. Section D, Biological crystallography, 50, 1994, pp. 225-227
Citations number
12
Categorie Soggetti
Crystallography,Biology,"Pharmacology & Pharmacy
Journal title
Acta crystallographica. Section D, Biological crystallography → ACNP
ISSN journal
09074449
Volume
50
Year of publication
1994
Part
2
Pages
225 - 227
Database
ISI
SICI code
0907-4449(1994)50:<225:CAPAOA>2.0.ZU;2-C
Abstract
Lipase from Chromobacterium viscosum has been purified to homogeneity and crystallized in a form suitable for X-ray diffraction analysis fro m 10-14% polyethylene glycol 4000 and 10-14% 2-methyl-2,4-pentane diol at pH 6.4 in the presence of 0.25%(w/v) n-octyl-beta-D-glucopyranosid e. These crystals belong to space group P2(1)2(1)2 with refined lattic e constants a = 41.1 angstrom, b = 156.8, c = 43.6 angstrom, indicatin g a cell content of one monomer per asymmetric unit of the crystal. Th e crystals diffract to a resolution of 2.2 angstrom.