MASS-SPECTROMETRIC STUDIES ON THE N-LINKED OLIGOSACCHARIDES OF BACULOVIRUS-EXPRESSED MOUSE INTERLEUKIN-3

Authors
HOGELAND KE DEINZER ML
Citation
Ke. Hogeland et Ml. Deinzer, MASS-SPECTROMETRIC STUDIES ON THE N-LINKED OLIGOSACCHARIDES OF BACULOVIRUS-EXPRESSED MOUSE INTERLEUKIN-3, Biological mass spectrometry, 23(4), 1994, pp. 218-224
Citations number
26
Categorie Soggetti
Spectroscopy,Biophysics
Journal title
Biological mass spectrometry → ACNP
ISSN journal
10529306
Volume
23
Issue
4
Year of publication
1994
Pages
218 - 224
Database
ISI
SICI code
1052-9306(1994)23:4<218:MSOTNO>2.0.ZU;2-0
Abstract
A combination of mass spectrometry techniques, high-pH anion-exchange chromatography and enzymatic digestions has been applied to elucidate the structures of the N-linked oligosaccharides on baculovirus-express ed mouse interleukin-3 produced in Bombyx mori larvae. This recombinan t lymphokine was found to be heterogeneously glycosylated with mannosi dic chitobiose core type oligosaccharides. A single N-linked oligosacc haride with the truncated structure of Man alpha 1-1-6Man beta 1-4GlcN Ac1-4(Fuc alpha 1-6)GlcNAc was found to be the predominant species.