REMOVAL OF THE OVARIAN SURFACE EPITHELIUM FROM THE RABBIT OVARY - A CAUSE OF ADHESIONS FOLLOWING A STANDARD INJURY

To assess the effect of the removal of ovarian surface epithelium on r epair, a standard injury was induced in the ovaries of 10 rabbits. In one ovary the surface cells were denuded, and in the other they were l eft intact. The effect on adhesion formation was assessed at 12 days. Adhesions were assessed by visual inspection at laparotomy and histolo gical examination of adhesion formation, including a stereological ass essment of scar volume. On visual assessment the overall adhesion scor es for the denuded ovaries were greater than for the intact ovaries. H istology showed the adhesions were attached only to the site of injury . The Fallopian tube was adherent to 35 and 4% of the denuded and inta ct ovarian segments respectively (P = 0.003). The scar volumes for eac h side were similar, After 12 days there was only partial re-epithelia lization on the denuded ovaries. Electron microscopy confirmed the slo w healing, with much of the surface still covered by a fibrinous-like exudate. The findings of this small study lend further weight to the i mportance of the surface epithelium in the control of adhesion formati on. Standard surgical procedures may generate adhesions by the inadver tent denuding of surface epithelium from adjacent healthy tissues, pos sibly by the loss of plasminogen activator activity that is found in t he mesothelium of the peritoneum. This study highlights the importance of controlling for inadvertent cell loss whilst investigating methods for adhesion prevention.