EXPRESSION OF FUNCTIONAL PROTEASE AND SUBVIRAL PARTICLES BY VACCINIA VIRUS CONTAINING EQUINE INFECTIOUS-ANEMIA VIRUS GAG AND 5' POL GENES

Authors
MCGUIRE TC OROURKE KI BASZLER TV LEIB SR BRASSFIELD AL DAVIS WC
Citation
Tc. Mcguire et al., EXPRESSION OF FUNCTIONAL PROTEASE AND SUBVIRAL PARTICLES BY VACCINIA VIRUS CONTAINING EQUINE INFECTIOUS-ANEMIA VIRUS GAG AND 5' POL GENES, Journal of General Virology, 75, 1994, pp. 895-900
Citations number
35
Categorie Soggetti
Virology
Journal title
Journal of General Virology → ACNP
ISSN journal
00221317
Volume
75
Year of publication
1994
Part
4
Pages
895 - 900
Database
ISI
SICI code
0022-1317(1994)75:<895:EOFPAS>2.0.ZU;2-K
Abstract
Cells infected with vaccinia viruses expressing the equine infectious anaemia virus (EIAV) gag gene (VGag) or gag plus the 5' pol encoding p rotease (VGag/PR) were evaluated with monoclonal antibody to a p26 cap sid protein linear epitope (QEISKFLTD). Both recombinant viruses expre ssed Gag precursor protein (55K) whereas only VGag/PR expressed a dete ctable Gag-Pol fusion protein (82K) with a functional protease, shown by subviral particles containing processed p26. Horses inoculated with VGag/PR produced antibodies reactive with EIAV Gag proteins.