ENGINEERING A UNIQUELY REACTIVE THIOL INTO A CYSTEINE-RICH PEPTIDE

Citation
E. Shimony et al., ENGINEERING A UNIQUELY REACTIVE THIOL INTO A CYSTEINE-RICH PEPTIDE, Protein engineering, 7(4), 1994, pp. 503-507
Citations number
25
Categorie Soggetti
Biology
Journal title
ISSN journal
02692139
Volume
7
Issue
4
Year of publication
1994
Pages
503 - 507
Database
ISI
SICI code
0269-2139(1994)7:4<503:EAURTI>2.0.ZU;2-#
Abstract
Cysteine mutagenesis for the purpose of chemical labelling was applied to the K+ channel neurotoxin charybdotoxin, a 37-residue peptide with six functionally essential cysteines. An additional 'spinster cystein e' was introduced at a position far away in space from the toxin's kno wn interaction surface where it contacts its K+ channel receptor. Desp ite the presence of the extra unpaired cysteine residue, the toxin sti ll folds efficiently and may be labelled by fluorescent and radioactiv e reagents to give a functionally competent toxin.