COMPARISON OF AMINO-ACID SEPARATIONS ON HIGH-PERFORMANCE SILICA-GEL, CELLULOSE, AND C-18 REVERSED-PHASE LAYERS AND APPLICATION OF HPTLC TO THE DETERMINATION OF AMINO-ACIDS IN BIOMPHALARIA-GLABRATA SNAILS

The separation of 18 amino acids on high performance silica gel, cellu lose, and reversed phase bonded silica gel plates has been compared. Z ones were detected with ninhydrin reagent. The ability to identify ami no acids in Biomphalaria. glabrata snail hemolymph and digestive gland -gonad complex (DGG) by HPTLC was studied, and alanine and aspartic ac id were quantified in hemolymph and DGG by scanning densitometry. Anal ytical results were compared to those reported earlier using column io n exchange and reversed phase liquid chromatography and paper chromato graphy.