A METHOD FOR QUICK DETERMINATION OF BILE-ACIDS IN BILE OF PATIENTS WITH BILIARY LITHIASIS

A single high-performance liquid chromatographic method is described f or determining and measuring the major conjugated bile acids in human bile. Four different chromatographic conditions were applied using c-1 8 columns and an isocratic solvent system. An effective one-step purif ication with Sep-pak C-18 was adopted. The advantages of this method a re an improved separation of the conjugated bile acids within a short period of time. The detection limit was 0.0125 mu g/mu l and linearity was up to 6 Recovery was always up to 96.6 %, this being sufficient f or routine clinical application during bile acids evaluation in biliar y lithiasis.