Jm. Degroodt et al., DETERMINATION OF IVERMECTIN RESIDUES IN MEAT AND LIVER BY HPLC AND FLUOROMETRIC DETECTION, Journal of liquid chromatography, 17(6), 1994, pp. 1419-1426
The method describes the analysis of ivermectin in meat and liver at a
quantification limit of 5 mu g/kg. This level is lower than the maxim
um residue limit (MRL) imposed for EEC countries. The absolute detecti
on limit was 250 pg. The recoveries ranged from 70 % to 88 %. Ivermect
in was extracted by a mixture of acetonitrile and water, purified on B
ond Elut C-18 Columns and converted to a fluorescent derivative using
trifluoroacetic anhydride and N-methylimidazol. The analysis was perfo
rmed on a liquid chromatograph fitted with a mu- Bondapak C-18 column
and ivermectin was detected by fluorescence spectroscopy. The method h
as already been used for routine analysis.