Xm. Zhang et al., CLOSTRIDIUM HYDROXYBENZOICUM SP-NOV, AN AMINO ACID-UTILIZING, HYDROXYBENZOATE-DECARBOXYLATING BACTERIUM ISOLATED FROM METHANOGENIC FRESH-WATER POND SEDIMENT, International journal of systematic bacteriology, 44(2), 1994, pp. 214-222
Clostridium hydroxybenzoicum JW/Z-1(T) (= ATCC 51151 = DSM 7310) (T =
type strain), isolated from freshwater pond sediment, is a nonmotile,
gram type-positive, spore-forming, amino acid-utilizing, anaerobic rod
. This bacterium produces two inducible enzymes that catalyze the deca
rboxylation of para-hydroxybenzoates. The phenols produced are not uti
lized. C. hydroxybenzoicum requires yeast extract for growth. Sugars a
re not utilized. Sodium ions and acetic acid stimulate growth. The opt
imal temperature and optimal pH for growth are 33 to 34 degrees C and
7.2 to 8.2, respectively. The DNA base composition of the type strain
is 35.5 mol% guanine plus cytosine, whereas the DNA base compositions
of the type strains of Clostridium sticklandii and Clostridium aminova
lericum are 33 and 33.5 mol% guanine plus cytosine, respectively, as d
etermined by a chemical method. 16S rRNA sequence analysis groups stra
in JW/Z-1(T) most closely with Clostridium purinolyticum and Clostridi
um acidiurici (10.6 and 11 inferred changes per 100 bases, respectivel
y). However, C. hydroxybenzoicum does not utilize uric acid, hypoxanth
ine, xanthine, adenine, or guanine. The cell wall type is A1 alpha (L-
Lys direct).