TRANSFER OF PSEUDOMONAS-PLANTARII AND PSEUDOMONAS-GLUMAE TO BURKHOLDERIA AS BURKHOLDERIA SPP AND DESCRIPTION OF BURKHOLDERIA-VANDII SP-NOV

Plant-associated bacteria were characterized and are discussed in rela tion to authentic members of the genus Pseudomonas sensu stricto. Bact eria belonging to Pseudomonas rRNA group I! are separated clearly from members of the genus Pseudomonas sensu stricto (Pseudomonas florescen s rRNA group) on the basis of plant association characteristics, chemo taxonomic characteristics, DNA-DNA hybridization data, rRNA-DNA hybrid ization data, and the sequences of 5S and 16S rRNAs. The transfer of P seudomonas cepacia, Pseudomonas mallei, Pseudomonas pseudomallei, Pseu domonas caryophylli, Pseudomonas gladioli, Pseudomonas pickettii, and Pseudomonas solanacearum to the new genus Burkholderia is supported; w e also propose that Pseudomonas plantarii and Pseudomonas glumae shoul d be transferred to the genus Burkholderia. Isolate VA-1316(T) (T = ty pe strain) was distinguished from Burkholderia species on the basis of physiological characteristics and DNA-DNA hybridization data. A new s pecies, Burkholderia vandii sp. nov. is proposed for this organism; th e type strain of B. vandii is VA-1316 (= JCM 7957).