PHYLOGENETIC-RELATIONSHIPS AMONG THE TRUE CITRUS-FRUIT TREES BY GLUTAMATE OXALOACETATE TRANSAMINASE ISOZYMES ANALYSIS

Leaf isozymes of glutamate oxaloaceate transaminase (EC 2.6.1.1; GOT) in the ''true citrus fruit trees'' group of Citrinae were analyzed usi ng Polyacrylamide gel electrophoresis to elucidate phylogenetic relati onships among them. Segregation at each of three loci, Got-1, Got-2 an d Got-3 of GOT isozymes in the progenies obtained from intergeneric cr osses between Citrus and Microcitrus species followed a simple Mendeli an inheritance, suggesting that Microcitrus and Citrus species have si milar genetic systems and the species of these two genera are closely related to each other. Some common alleles at the three loci controlli ng GOT isozymes were recognized between the genera of ''true citrus fr uit trees''. These findings suggest that a genetic identity is conserv ed among the ''true citrus fruit trees'' group; even some of them clim atically, geographically and reproductively isolated.